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anti mouse plgf polyclonal antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti mouse plgf polyclonal antibody
    Anti Mouse Plgf Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+plgf+polyclonal+antibody/Mouse+PlGF-2+Antibody/pm23122125-112-114-118
    Average 93 stars, based on 4 article reviews
    anti mouse plgf polyclonal antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Dietary Monascus adlay supplements facilitate suppression of cigarette smoke-induced pulmonary endoplasmic reticulum stress, autophagy, apoptosis and emphysema-related PLGF in the rat.
    Article Snippet: Cigarette smoke (CS) exposure may cause oxidative stress in the lung, leading to cell death and long-term injury.. Monascus adlay (MA) with antioxidant components produced by inoculating adlay (Cois lachrymal-jobi L. var. ma-yuen Stapf) with Monascus purpureus may protect lung against CS-induced lung injuries in rats.. MA and lovastatin had higher antioxidant activities than either M. purpureus or adlay.

    Transduction:

    Article Title: Dietary Monascus adlay supplements facilitate suppression of cigarette smoke-induced pulmonary endoplasmic reticulum stress, autophagy, apoptosis and emphysema-related PLGF in the rat.
    Article Snippet: Cigarette smoke (CS) exposure may cause oxidative stress in the lung, leading to cell death and long-term injury.. Monascus adlay (MA) with antioxidant components produced by inoculating adlay (Cois lachrymal-jobi L. var. ma-yuen Stapf) with Monascus purpureus may protect lung against CS-induced lung injuries in rats.. MA and lovastatin had higher antioxidant activities than either M. purpureus or adlay.

    Activation Assay:

    Article Title: Dietary Monascus adlay supplements facilitate suppression of cigarette smoke-induced pulmonary endoplasmic reticulum stress, autophagy, apoptosis and emphysema-related PLGF in the rat.
    Article Snippet: Cigarette smoke (CS) exposure may cause oxidative stress in the lung, leading to cell death and long-term injury.. Monascus adlay (MA) with antioxidant components produced by inoculating adlay (Cois lachrymal-jobi L. var. ma-yuen Stapf) with Monascus purpureus may protect lung against CS-induced lung injuries in rats.. MA and lovastatin had higher antioxidant activities than either M. purpureus or adlay.

    Binding Assay:

    Article Title: Dietary Monascus adlay supplements facilitate suppression of cigarette smoke-induced pulmonary endoplasmic reticulum stress, autophagy, apoptosis and emphysema-related PLGF in the rat.
    Article Snippet: Cigarette smoke (CS) exposure may cause oxidative stress in the lung, leading to cell death and long-term injury.. Monascus adlay (MA) with antioxidant components produced by inoculating adlay (Cois lachrymal-jobi L. var. ma-yuen Stapf) with Monascus purpureus may protect lung against CS-induced lung injuries in rats.. MA and lovastatin had higher antioxidant activities than either M. purpureus or adlay.

    Immunoprecipitation:

    Article Title: Mice overexpressing placenta growth factor exhibit increased vascularization and vessel permeability.
    Article Snippet: .. Two 6 mm diameter punch biopsies were homogenized in RIPA buffer (150 mM NaCl, 1% NP-40, 0.1% SDS, 50 mM Tris-HCl pH 7.5) and immunoprecipitated using an anti-mouse PlGF polyclonal antibody (AF 465, R&D Systems) as previously described (Failla et al., 2000). .. 100 ng of mouse recombinant PlGF-2 (R&D Systems) were also immunoprecipitated as a positive control.

    Injection:

    Article Title: Role of vascular endothelial growth factor and placental growth factors during retinal vascular development and hyaloid regression.
    Article Snippet: PURPOSE.. Vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF) are members of a large group of homologous peptides identified as the VEGF family.. Although VEGF-A is known to act as a potent angiogenic peptide in the retina, the vasoactive function of PlGF in this tissue is less well defined.

    Sterility:

    Article Title: Role of vascular endothelial growth factor and placental growth factors during retinal vascular development and hyaloid regression.
    Article Snippet: PURPOSE.. Vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF) are members of a large group of homologous peptides identified as the VEGF family.. Although VEGF-A is known to act as a potent angiogenic peptide in the retina, the vasoactive function of PlGF in this tissue is less well defined.



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    Figure 3. Immunohistochemical analyses of HIF-1 and <t>PLGF</t> expression. Tissue was harvested from AdCA5- and AdLacZ- injected eyes 24 hours or 6 days after subretinal injection, and sections were stained with antibodies against HIF-1 or PLGF, respectively. HIF-1 expression is observed at the site of AdCA5 injection (arrows). In AdCA5-injected eyes, PLGF expres- sion is prominent in the INL (vertical arrows) with vertical lines in the inner plexiform layer (horizontal arrows) typical of Muller cell processes and irregular horizontal linear-stained structures near the surface of the retina (arrowheads) typical of Muller cell end feet. In AdLacZ-injected eyes, modest PLGF staining is observed in the INL and near the retinal surface.
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    Figure 3. Immunohistochemical analyses of HIF-1 and <t>PLGF</t> expression. Tissue was harvested from AdCA5- and AdLacZ- injected eyes 24 hours or 6 days after subretinal injection, and sections were stained with antibodies against HIF-1 or PLGF, respectively. HIF-1 expression is observed at the site of AdCA5 injection (arrows). In AdCA5-injected eyes, PLGF expres- sion is prominent in the INL (vertical arrows) with vertical lines in the inner plexiform layer (horizontal arrows) typical of Muller cell processes and irregular horizontal linear-stained structures near the surface of the retina (arrowheads) typical of Muller cell end feet. In AdLacZ-injected eyes, modest PLGF staining is observed in the INL and near the retinal surface.
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    Figure 3. Immunohistochemical analyses of HIF-1 and <t>PLGF</t> expression. Tissue was harvested from AdCA5- and AdLacZ- injected eyes 24 hours or 6 days after subretinal injection, and sections were stained with antibodies against HIF-1 or PLGF, respectively. HIF-1 expression is observed at the site of AdCA5 injection (arrows). In AdCA5-injected eyes, PLGF expres- sion is prominent in the INL (vertical arrows) with vertical lines in the inner plexiform layer (horizontal arrows) typical of Muller cell processes and irregular horizontal linear-stained structures near the surface of the retina (arrowheads) typical of Muller cell end feet. In AdLacZ-injected eyes, modest PLGF staining is observed in the INL and near the retinal surface.
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    Figure 3. Immunohistochemical analyses of HIF-1 and <t>PLGF</t> expression. Tissue was harvested from AdCA5- and AdLacZ- injected eyes 24 hours or 6 days after subretinal injection, and sections were stained with antibodies against HIF-1 or PLGF, respectively. HIF-1 expression is observed at the site of AdCA5 injection (arrows). In AdCA5-injected eyes, PLGF expres- sion is prominent in the INL (vertical arrows) with vertical lines in the inner plexiform layer (horizontal arrows) typical of Muller cell processes and irregular horizontal linear-stained structures near the surface of the retina (arrowheads) typical of Muller cell end feet. In AdLacZ-injected eyes, modest PLGF staining is observed in the INL and near the retinal surface.
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    Image Search Results


    Figure 3. Immunohistochemical analyses of HIF-1 and PLGF expression. Tissue was harvested from AdCA5- and AdLacZ- injected eyes 24 hours or 6 days after subretinal injection, and sections were stained with antibodies against HIF-1 or PLGF, respectively. HIF-1 expression is observed at the site of AdCA5 injection (arrows). In AdCA5-injected eyes, PLGF expres- sion is prominent in the INL (vertical arrows) with vertical lines in the inner plexiform layer (horizontal arrows) typical of Muller cell processes and irregular horizontal linear-stained structures near the surface of the retina (arrowheads) typical of Muller cell end feet. In AdLacZ-injected eyes, modest PLGF staining is observed in the INL and near the retinal surface.

    Journal: Circulation Research

    Article Title: Cell Type–Specific Regulation of Angiogenic Growth Factor Gene Expression and Induction of Angiogenesis in Nonischemic Tissue by a Constitutively Active Form of Hypoxia-Inducible Factor 1

    doi: 10.1161/01.res.0000102937.50486.1b

    Figure Lengend Snippet: Figure 3. Immunohistochemical analyses of HIF-1 and PLGF expression. Tissue was harvested from AdCA5- and AdLacZ- injected eyes 24 hours or 6 days after subretinal injection, and sections were stained with antibodies against HIF-1 or PLGF, respectively. HIF-1 expression is observed at the site of AdCA5 injection (arrows). In AdCA5-injected eyes, PLGF expres- sion is prominent in the INL (vertical arrows) with vertical lines in the inner plexiform layer (horizontal arrows) typical of Muller cell processes and irregular horizontal linear-stained structures near the surface of the retina (arrowheads) typical of Muller cell end feet. In AdLacZ-injected eyes, modest PLGF staining is observed in the INL and near the retinal surface.

    Article Snippet: To detect PLGF expression, sections were preincubated with a blocking solution of 1% skim milk in TBS for 30 minutes and incubated overnight with a 1:20 dilution of a polyclonal goat anti-mouse PLGF antibody (Santa Cruz Biotechnology, Santa Cruz, Calif) in blocking solution.

    Techniques: Immunohistochemical staining, Expressing, Injection, Staining

    Figure 7. Regulation of angiogenesis by HIF-1. In a cell type– specific manner, HIF-1 can either activate (arrow) or repress (blocked arrow) expression of genes encoding angiogenic growth factors (PLGF, VEGF, ANGPT1, ANGPT2, and PDGFB) that bind to receptors (VEGFR1, VEGFR2, TIE2, and PDGFR) on vascular ECs or SMCs. Receptor signaling leads to critical events required for physiological angiogenesis.

    Journal: Circulation Research

    Article Title: Cell Type–Specific Regulation of Angiogenic Growth Factor Gene Expression and Induction of Angiogenesis in Nonischemic Tissue by a Constitutively Active Form of Hypoxia-Inducible Factor 1

    doi: 10.1161/01.res.0000102937.50486.1b

    Figure Lengend Snippet: Figure 7. Regulation of angiogenesis by HIF-1. In a cell type– specific manner, HIF-1 can either activate (arrow) or repress (blocked arrow) expression of genes encoding angiogenic growth factors (PLGF, VEGF, ANGPT1, ANGPT2, and PDGFB) that bind to receptors (VEGFR1, VEGFR2, TIE2, and PDGFR) on vascular ECs or SMCs. Receptor signaling leads to critical events required for physiological angiogenesis.

    Article Snippet: To detect PLGF expression, sections were preincubated with a blocking solution of 1% skim milk in TBS for 30 minutes and incubated overnight with a 1:20 dilution of a polyclonal goat anti-mouse PLGF antibody (Santa Cruz Biotechnology, Santa Cruz, Calif) in blocking solution.

    Techniques: Expressing

    Figure 6. Gene expression after intravitreous adenoviral injection. Total RNA was isolated from whole eyes at the indicated time after intravit- reous injection of AdCA5 and AdLacZ. The fold induction of HIF-1, PLGF, VEGF, ANGPT1, ANGPT2, and PDGFB mRNA in the AdCA5- injected eye relative to the AdLacZ-injected eye of each mouse was determined by real-time RT-PCR. Mean and SE (n3 to 5) are shown.

    Journal: Circulation Research

    Article Title: Cell Type–Specific Regulation of Angiogenic Growth Factor Gene Expression and Induction of Angiogenesis in Nonischemic Tissue by a Constitutively Active Form of Hypoxia-Inducible Factor 1

    doi: 10.1161/01.res.0000102937.50486.1b

    Figure Lengend Snippet: Figure 6. Gene expression after intravitreous adenoviral injection. Total RNA was isolated from whole eyes at the indicated time after intravit- reous injection of AdCA5 and AdLacZ. The fold induction of HIF-1, PLGF, VEGF, ANGPT1, ANGPT2, and PDGFB mRNA in the AdCA5- injected eye relative to the AdLacZ-injected eye of each mouse was determined by real-time RT-PCR. Mean and SE (n3 to 5) are shown.

    Article Snippet: To detect PLGF expression, sections were preincubated with a blocking solution of 1% skim milk in TBS for 30 minutes and incubated overnight with a 1:20 dilution of a polyclonal goat anti-mouse PLGF antibody (Santa Cruz Biotechnology, Santa Cruz, Calif) in blocking solution.

    Techniques: Gene Expression, Injection, Isolation, Quantitative RT-PCR